Large-Scale Gradient Elution Chromatography
نویسندگان
چکیده
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منابع مشابه
Modeling of Gradient Elution in Multicomponent Nonlinear Chromatography
-Gradient elution in nonlinear chromatography is a very powerful tool for the separation of components having a wide range of retentivity. It is not only used for chemical analysis, but also used in preparativeand large-scale chromatography for the separation of many macromolecules, such as proteins. In this work, a general rate model has been presented for the study of gradient elution in nonl...
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Here we describe fast analysis of biomolecules by newly developed pressure-driven liquid chromatography (LC) on a chip with integration of a gradient elution system. We have reported the fabrication of a gradient elution system using a cross-Tesla mixer on a chip with pillar array columns [1]. In this study, we applied the chip to the analysis of biological compounds, and showed that 10 times f...
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A gradient elution high performance liquid chromatography method with detection at 230 nm for the analysis of perbenzoylated cerebrosides containing hydroxy and nonhydroxy fatty acids is described. The quantitative range of the method is 0.5-10 nmol of cerebrosides. The limit of detection for injected sample is about 10 picomol. The analysis time by liquid chromatography is less than 5 minutes ...
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Chromatographers are cautioned to avoid gradient elution when isocratic elution will do. In this work, we compared the analytical properties of gradient and isocratic separations of a sample which can be done quite readily under isocratic conditions. We found that gradient elution gave a shorter overall analysis with similar resolution of the critical pair compared to isocratic elution without ...
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This paper reports a gradient elution system for pressure-driven liquid chromatography (LC) on a chip. We have already reported the microfabrication of efficient pillar array columns with low dispersion turns. For faster and more efficient analysis, we fabricated a gradient elution system with a cross-Tesla mixer for the effective mixing of two solutions on a chip with a separation channel of p...
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